Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
To Be Published
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Phalloidin. (Complex from Amanita phalloides)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Actin filament (Complex from Homo sapiens)
- Phosphate ion (94 Da, Ligand)
Cytoplasmic dynein-1 A1/A2 motor domains bound to LIS1
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 8pqz
Q-score: 0.284
Singh K, Lau CK, Manigrasso G, Gama JB, Gassmann R, Carter AP
Science (2024) 383 pp. eadk8544-eadk8544 [ Pubmed: 38547289 DOI: doi:10.1126/science.adk8544 ]
- Cytoplasmic dynein 1 intermediate chain 2 (68 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Platelet-activating factor acetylhydrolase ib subunit beta (46 kDa, Protein from Homo sapiens)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cytoplasmic dynein 1 heavy chain 1 (533 kDa, Protein from Homo sapiens)
- Cytoplasmic dynein-a1/a2 motor domains bound to lis1 (Complex from Homo sapiens)
Apo KIF20A[1-565] class-2 in complex with a microtubule
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 8f18
Q-score: 0.404
Ranaivoson FM, Crozet V, Benoit MPMH, Abdalla Mohammed Khalid A, Kikuti C, Sirkia H, El Marjou A, Miserey-Lenkei S, Asenjo AB, Sosa H, Schmidt CF, Rosenfeld SS, Houdusse A
Open Biol (2023) 13 pp. 230122-230122 [ DOI: doi:10.1098/rsob.230122 Pubmed: 37726093 ]
- Kif20a[1-565] apo (Complex from Mus musculus)
- Kinesin-like protein kif20a (64 kDa, Protein from Mus musculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 15r microtubule (Cellular component from Sus scrofa)
- Taxol (853 Da, Ligand)
- Kif20a[1-565] apo in complex with a microtubule - class 2 (Complex)
- Tubulin beta-2b chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
Apo KIF20A[1-565] class-1 in complex with a microtubule
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 8f1a
Q-score: 0.463
Ranaivoson FM, Crozet V, Benoit MPMH, Abdalla Mohammed Khalid A, Kikuti C, Sirkia H, El Marjou A, Miserey-Lenkei S, Asenjo AB, Sosa H, Schmidt CF, Rosenfeld SS, Houdusse A
Open Biol (2023) 13 pp. 230122-230122 [ DOI: doi:10.1098/rsob.230122 Pubmed: 37726093 ]
- Kif20a[1-565] apo in complex with a microtubule (Complex)
- Kif20a[1-565] apo (Complex from Mus musculus)
- Kinesin-like protein kif20a (64 kDa, Protein from Mus musculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 15r microtubule (Cellular component from Sus scrofa)
- Taxol (853 Da, Ligand)
- Tubulin beta-2b chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
Cryo-EM structure of ADP-bound, filamentous beta-actin harboring the R183W mutation
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 8oi8
Q-score: 0.679
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ DOI: doi:10.1038/s41594-023-01101-9 Pubmed: 37749275 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Actin filament harboring the r183w mutation. (Complex from Homo sapiens)
Cryo-EM structure of ADP-bound, filamentous beta-actin harboring the N111S mutation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8oid
Q-score: 0.681
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ DOI: doi:10.1038/s41594-023-01101-9 Pubmed: 37749275 ]
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Water (18 Da, Ligand)
- Actin filament harboring the n111s mutation. (Complex from Homo sapiens)
Cryo-EM structure of alpha-synuclein singlet filament from Juvenile-onset synucleinopathy
Resolution: 2.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 8bqv
Q-score: 0.74
Yang Y, Garringer HJ, Shi Y, Lovestam S, Peak-Chew S, Zhang X, Kotecha A, Bacioglu M, Koto A, Takao M, Spillantini MG, Ghetti B, Vidal R, Murzin AG, Scheres SHW, Goedert M
Acta Neuropathol (2023) 145 pp. 561-572 [ DOI: doi:10.1007/s00401-023-02550-8 Pubmed: 36847833 ]
- Alpha-synuclein (14 kDa, Protein from human)
- Water (18 Da, Ligand)
- Alpha-synuclein singlet filament extracted from the human brain with jos (Tissue from Homo sapiens)
- Unknown protein (613 Da, Protein from Human)
Cryo-EM structure of alpha-synuclein filaments doublet from Juvenile-onset synucleinopathy
Resolution: 2.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 8bqw
Q-score: 0.652
Yang Y, Garringer HJ, Shi Y, Lovestam S, Peak-Chew S, Zhang X, Kotecha A, Bacioglu M, Koto A, Takao M, Spillantini MG, Ghetti B, Vidal R, Murzin AG, Scheres SHW, Goedert M
Acta Neuropathol (2023) 145 pp. 561-572 [ DOI: doi:10.1007/s00401-023-02550-8 Pubmed: 36847833 ]
- Alpha-synuclein (14 kDa, Protein from human)
- Alpha-synuclein doublet filament extracted from the human brain with jos (Tissue from Homo sapiens)
- Water (18 Da, Ligand)
- Unknown protein (783 Da, Protein from Human)
Non-muscle F-actin decorated with non-muscle tropomyosin 1.6
Resolution: 3.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 7ztc
Q-score: 0.42
Selvaraj M, Kokate SB, Reggiano G, Kogan K, Kotila T, Kremneva E, DiMaio F, Lappalainen P, Huiskonen JT
Cell Rep (2023) 42 pp. 111900-111900 [ DOI: doi:10.1016/j.celrep.2022.111900 Pubmed: 36586407 ]
- Beta actin (non-muscle) (Cellular component from Homo sapiens)
- Non-muscle tropomyosin 1.6 (Cellular component from Homo sapiens)
- Actin, cytoplasmic 1 (41 kDa, Protein from human)
- Non-muscle tropomyosin 1.6 (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Non-muscle f-actin decorated with non-muscle tropomyosin 1.6 (Complex from Homo sapiens)
Non-muscle F-actin decorated with non-muscle tropomyosin 3.2
Resolution: 4.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7ztd
Q-score: 0.36
Selvaraj M, Kokate SB, Reggiano G, Kogan K, Kotila T, Kremneva E, DiMaio F, Lappalainen P, Huiskonen JT
Cell Rep (2023) 42 pp. 111900-111900 [ DOI: doi:10.1016/j.celrep.2022.111900 Pubmed: 36586407 ]
- Beta actin (non-muscle) (Cellular component from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from human)
- Non-muscle f-actin decorated with non-muscle tropomyosin 3.2 (Complex from Homo sapiens)
- Non-muscle tropomyosin 3.2 (Cellular component from Homo sapiens)
- Non-muscle tropomyosin 3.2 (11 kDa, Protein from Homo sapiens)